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lördag 25 januari 2020

PI4L IIIbeta

https://www.ncbi.nlm.nih.gov/pubmed/22253445

2012 Mar 9;287(11):8457-67. doi: 10.1074/jbc.M111.312561. Epub 2012 Jan 17.

Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.

Abstract

Phosphatidylinositol kinases (PI kinases) play an important role in the life cycle of several viruses after infection. Using gene knockdown technology, we demonstrate that phosphatidylinositol 4-kinase IIIβ (PI4KB) is required for cellular entry by pseudoviruses bearing the severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein and that the cell entry mediated by SARS-CoV spike protein is strongly inhibited by knockdown of PI4KB. Consistent with this observation, pharmacological inhibitors of PI4KB blocked entry of SARS pseudovirions. Further research suggested that PI4P plays an essential role in SARS-CoV spike-mediated entry, which is regulated by the PI4P lipid microenvironment. We further demonstrate that PI4KB does not affect virus entry at the SARS-CoV S-ACE2 binding interface or at the stage of virus internalization but rather at or before virus fusion. Taken together, these results indicate a new function for PI4KB and suggest a new drug target for preventing SARS-CoV infection.
PMID:
22253445
PMCID:
PMC3318727
DOI:
10.1074/jbc.M111.312561


Joitain PI4KIIIbeta inhibittoreita on olemassa.
 https://www.sciencedirect.com/science/article/pii/S0960894X14007392?via%3Dihub