WIPI2(7p22.1). Atg21, ATG18B, CGI-50, IDDSSA. WD-toistojaksoinen fosfoinositidin kanssa interaktion tekevä proteiini2

https://www.ncbi.nlm.nih.gov/gene/26100

Also known as

Atg21; ATG18B; CGI-50; IDDSSA; WIPI-2

Summary

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]

Expression

Ubiquitous expression in testis (RPKM 20.8), brain (RPKM 12.7) and 25 other tissues See more

Orthologs mouse all

Preferred Names

WD repeat domain phosphoinositide-interacting protein 2

Names

WD40 repeat protein interacting with phosphoinositides 2

WIPI49-like protein 2

                     /db_xref="MIM:609225"
ORIGIN      
        1 mnlasqsgea gagqllfanf nqdntevkga sraaglgrra vvwslavgsk sgykffslss
       61 vdkleqiyec tdtedvcive rlfssslvai vslkaprklk vchfkkgtei cnysysntil
      121 avklnrqrli vcleeslyih nirdmkvlht iretppnpag lcalsinndn cylaypgsat
      181 igevqvfdti nlraanmipa hdsplaalaf dasgtklata sekgtvirvf sipegqklfe
      241 frrgvkrcvs icslafsmdg mflsassnte tvhifkletv kekppeeptt wtgyfgkvlm
      301 astsylpsqv temfnqgraf atvrlpfcgh knicslatiq kiprllvgaa dgylymynld
      361 pqeggecalm kqhrldgsle ttneildsas hdcplvtqty gaaagkaytd dlgavggacl
      421 edeasalrld edsehppmil rtd

 Conserved Domains (3) summary

COG2319
Location:37 → 290

WD40; WD40 repeat [General function prediction only]

sd00039
Location:161 → 200

7WD40; WD40 repeat [structural motif]

cl02567
Location:184 → 363

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from .

Related articles in PubMed

1. mTORC1-Regulated and HUWE1-Mediated WIPI2 Degradation Controls Autophagy Flux. Wan W, et al. Mol Cell, 2018 Oct 18. PMID 30340022
2. Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy. Thurston TL, et al. EMBO J, 2016 Aug 15. PMID 27370208, Free PMC Article
3. Fluorescence-based imaging of autophagy progression by human WIPI protein detection. Thost AK, et al. Methods, 2015 Mar. PMID 25462558
4. WIPI β-propellers at the crossroads of autophagosome and lipid droplet dynamics. Pfisterer SG, et al. Biochem Soc Trans, 2014 Oct. PMID 25233424
5. WIPI2b and Atg16L1: setting the stage for autophagosome formation. Wilson MI, et al. Biochem Soc Trans, 2014 Oct. PMID 25233411

See all (41) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

1. Regulation of the intracellular WIPI2 protein level by mTORC1 and HUWE1 is a key determinant of autophagy flux.
2. Results suggest that Optn potentiates LC3-II production and maturation of the phagophore into the autophagosome, by facilitating the recruitment of the Atg12-5-16L1 complex to Wipi2-positive phagophores.
3. Data suggest that, in patients with diabetic kidney disease, urinary excretion of mRNAs for MAP1LC3A, WIPI2, and RB1CC1 is down-regulated as compared to healthy control subjects; these transcripts may serve as urinary autophagy biomarkers. (MAP1LC3A = microtubule associated protein 1 light chain 3; WIPI2 = WD repeat domain phosphoinositide-interacting protein 2; RB1CC1 = RB1 inducible coiled-coil 1)
4. Here the authors show that recruitment of WIPI2, itself essential for anti-bacterial autophagy, is dependent on the localization of catalytically active TBK1 to the vicinity of cytosolic bacteria.
5. The specific autophagosomal localization of both WIPI1 and WIPI2 (refered to as WIPI puncta) has been employed to assess autophagy using fluorescence microscopy methods, such as confocal and live-cell video microscopy
6. Data suggest WIPI1/WIPI2 co-localize with microtubule-associated light chain 3 and autophagy related proteins 2/14L, participate in biogenesis of phagosomes, autophagy, and mobilization of lipids to/from intracellular droplets. [review-like article]
7. WIPI-1 and WIPI-2 are functionally required in mediating the PI3P signal at the onset of autophagy in NB4 cells.
8. Data suggest that WIPI2b directly interacts with dimer of ATG16L1 (autophagy related 16-like 1) and this interaction is linked to production of phosphatidylinositol 3-phosphate in endoplasmic reticulum triggered by autophagosome formation. [REVIEW]
9. WIPI2b binds the membrane surrounding Salmonella and recruits the Atg12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.
10. A role for WIPI2 in the progression of omegasomes into autophagosomes, is reported.

Submit: New GeneRIF Correction See all GeneRIFs (12)