PI3P synteesi , VPS
15 ja VPS34 , mitä ne tekevät vakuolaaristen proteiinien
lajitelujärjstlmään assosioituneina?
Vacuolar Protein Sorting Associated protein 15
VPS15 (Kr. 3q22.3),
PIK3R4, Phosphoinositide-3-kinase regulatory subunit 4.
- Also known as p150; VPS15
- Expression Ubiquitous expression in thyroid (RPKM 9.2), testis (RPKM 8.7) and 25 other tissues See moreOrthologs mouse all
Preferred Names
- phosphoinositide 3-kinase regulatory subunit 4
- Names
- PI3-kinase p150 subunit
- PI3-kinase regulatory subunit 4
- phosphatidylinositol 3-kinase-associated p150
- phosphoinositide 3-kinase adaptor protein
- phosphoinositide-3-kinase, regulatory subunit 4, p150
- NP_055417.1
-
- EC 2.7.11.1
Peptidirakenne:
- Conserved Domains (8) summary
- smart00320
Location:1327 → 1358 - WD40; WD40 repeats
-
smart00220
Location:26 → 309 - S_TKc; Serine/Threonine protein kinases, catalytic domain
-
COG2319
Location:991 → 1358 - WD40; WD40 repeat [General function prediction only]
-
cd13980
Location:25 → 320 - STKc_Vps15; Catalytic domain of the Serine/Threonine kinase, Vacuolar protein sorting-associated protein 15
-
pfam07539
Location:352 → 448 - DRIM; Down-regulated in metastasis
-
sd00044
Location:539 → 566 - HEAT; HEAT repeat [structural motif]
-
sd00039
Location:997 → 1040 - 7WD40; WD40 repeat [structural motif]
-
cl02567
Location:985 → 1269 - WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
Related articles in PubMed
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Autophagy dysregulation in Danon disease. Nascimbeni AC, et al. Cell Death Dis, 2017 Jan 19. PMID 28102838, Free PMC Article
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High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia. Kristensen L, et al. Leuk Res, 2015 Jun. PMID 25840748
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hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells. Yan Y, et al. Biochem J, 2009 Feb 1. PMID 18957027, Free PMC Article
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The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Ligon LA, et al. Mol Biol Cell, 2003 Apr. PMID 12686597, Free PMC Article
-
Signal
transduction pathways mediated by the interaction of CpG DNA with
Toll-like receptor 9. Takeshita F, et al. Semin
Immunol, 2004 Feb. PMID 14751759
See citations in PubMed for homologs of this gene provided by HomoloGene
GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?
Rakenne ja historia
phosphoinositide
3-kinase regulatory subunit 4 [Homo sapiens]
NCBI Reference Sequence: NP_055417.1Identical Proteins FASTA Graphics
LOCUS NP_055417 1358 aa linear PRI 01-APR-2018 DEFINITION phosphoinositide 3-kinase regulatory subunit 4 [Homo sapiens]. ACCESSION NP_055417 VERSION NP_055417.1 DBSOURCE REFSEQ: accession NM_014602.2 KEYWORDS RefSeq. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (residues 1 to 1358) AUTHORS Nascimbeni AC, Fanin M, Angelini C and Sandri M. TITLE Autophagy dysregulation in Danon disease JOURNAL Cell Death Dis 8 (1), e2565 (2017) PUBMED 28102838 REMARK GeneRIF: Both Danon Disease and glycogen storage disease type II show accumulation and altered localization of VPS15 in autophagy-incompetent fibers. However, TFEB displays a different pattern between these two lysosomal storage diseases Publication Status: Online-Only REFERENCE 2 (residues 1 to 1358) AUTHORS Kristensen L, Kristensen T, Abildgaard N, Thomassen M, Frederiksen M, Mour phosphoinositide 3-kinase regulatory subunit 4 [Homo sapiens] NCBI Reference Sequence: NP_055417.1 Identical Proteins FASTA Graphics Go to: LOCUS NP_055417 1358 aa linear PRI 01-APR-2018 DEFINITION phosphoinositide 3-kinase regulatory subunit 4 [Homo sapiens]. ACCESSION NP_055417 VERSION NP_055417.1 DBSOURCE REFSEQ: accession NM_014602.2 KEYWORDS RefSeq. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (residues 1 to 1358) AUTHORS Nascimbeni AC, Fanin M, Angelini C and Sandri M. TITLE Autophagy dysregulation in Danon disease JOURNAL Cell Death Dis 8 (1), e2565 (2017) PUBMED 28102838 REMARK GeneRIF: Both Danon Disease and glycogen storage disease type II show accumulation and altered localization of VPS15 in autophagy-incompetent fibers. However, TFEB displays a different pattern between these two lysosomal storage diseases Publication Status: Online-Only REFERENCE 2 (residues 1 to 1358) AUTHORS Kristensen L, Kristensen T, Abildgaard N, Thomassen M, Frederiksen M, Mourits-Andersen T and Moller MB. TITLE High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia JOURNAL Leuk. Res. 39 (6), 555-560 (2015) PUBMED 25840748 REMARK GeneRIF: High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia. REFERENCE 3 (residues 1 to 1358) AUTHORS Cao Y, Wang Y, Abi Saab WF, Yang F, Pessin JE and Backer JM. TITLE NRBF2 regulates macroautophagy as a component of Vps34 Complex I JOURNAL Biochem. J. 461 (2), 315-322 (2014) PUBMED 24785657 REFERENCE 4 (residues 1 to 1358) AUTHORS Lu J, He L, Behrends C, Araki M, Araki K, Jun Wang Q, Catanzaro JM, Friedman SL, Zong WX, Fiel MI, Li M and Yue Z. TITLE NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity JOURNAL Nat Commun 5, 3920 (2014) PUBMED 24849286 REMARK Publication Status: Online-Only REFERENCE 5 (residues 1 to 1358) AUTHORS Fogel AI, Dlouhy BJ, Wang C, Ryu SW, Neutzner A, Hasson SA, Sideris DP, Abeliovich H and Youle RJ. TITLE Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy JOURNAL Mol. Cell. Biol. 33 (18), 3675-3688 (2013) PUBMED 23878393 REFERENCE 6 (residues 1 to 1358) AUTHORS Stein MP, Feng Y, Cooper KL, Welford AM and Wandinger-Ness A. TITLE Human VPS34 and p150 are Rab7 interacting partners JOURNAL Traffic 4 (11), 754-771 (2003) PUBMED 14617358 REFERENCE 7 (residues 1 to 1358) AUTHORS Ligon LA, Shelly SS, Tokito M and Holzbaur EL. TITLE The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization JOURNAL Mol. Biol. Cell 14 (4), 1405-1417 (2003) PUBMED 12686597 REFERENCE 8 (residues 1 to 1358) AUTHORS Koyasu S. TITLE The role of PI3K in immune cells JOURNAL Nat. Immunol. 4 (4), 313-319 (2003) PUBMED 12660731 REMARK Review article REFERENCE 9 (residues 1 to 1358) AUTHORS Askham JM, Vaughan KT, Goodson HV and Morrison EE. TITLE Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome JOURNAL Mol. Biol. Cell 13 (10), 3627-3645 (2002) PUBMED 12388762 REFERENCE 10 (residues 1 to 1358) AUTHORS Panaretou C, Domin J, Cockcroft S and Waterfield MD. TITLE Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex JOURNAL J. Biol. Chem. 272 (4), 2477-2485 (1997) PUBMED 8999962 COMMENT VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP359657.1 and Y08991.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y08991.1, SRR1803616.271117.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## FEATURES Location/Qualifiers source 1..1358 /organism="Homo sapiens" /db_xref="taxon:9606" /chromosome="3" /map="3q22.1" Protein 1..1358 /product="phosphoinositide 3-kinase regulatory subunit 4" /EC_number="2.7.11.1" /note="phosphatidylinositol 3-kinase-associated p150; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase regulatory subunit 4; PI3-kinase p150 subunit; phosphoinositide 3-kinase adaptor protein" /calculated_mol_wt=152973 Region 25..320 /region_name="STKc_Vps15" /note="Catalytic domain of the Serine/Threonine kinase, Vacuolar protein sorting-associated protein 15; cd13980" /db_xref="CDD:270882" Region 26..309 /region_name="S_TKc" /note="Serine/Threonine protein kinases, catalytic domain; smart00220" /db_xref="CDD:214567" Site order(32..36,40,51,53,85,103..106,109,111,148,150, 152..153,155,166,169,192..195) /site_type="active" /db_xref="CDD:270882" Site order(32..36,40,51,53,85,103..106,109,148,150,152..153, 155,166) /site_type="other" /note="ATP binding site [chemical binding]" /db_xref="CDD:270882" Site order(36,109,111,148,150,152,169,192..195) /site_type="other" /note="polypeptide substrate binding site [polypeptide binding]" /db_xref="CDD:270882" Site order(165..187,192..195) /site_type="other" /note="activation loop (A-loop)" /db_xref="CDD:270882" Region <352 ..="">448 /region_name="DRIM" /note="Down-regulated in metastasis; pfam07539" /db_xref="CDD:284870" Region 377..408 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Region 413..450 /region_name="HEAT 1" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 417..443 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Region 458..495 /region_name="HEAT 2" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 460..489 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Region 539..566 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Site order(554..555,558,562..563,566,595..596,599,602..603,606, 633..634,637,640..641,673..674,677,680..681) /site_type="other" /note="putative peptide binding site [polypeptide binding]" /db_xref="CDD:293787" Region 572..610 /region_name="HEAT 3" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 576..607 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Region 614..641 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Region 652..682 /region_name="HEAT repeat" /note="HEAT repeat [structural motif]" /db_xref="CDD:293787" Site 808 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine. {ECO:0000244|PubMed:19369195}; propagated from UniProtKB/Swiss-Prot (Q99570.3)" Site 813 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine. {ECO:0000244|PubMed:23186163}; propagated from UniProtKB/Swiss-Prot (Q99570.3)" Site 853 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine. {ECO:0000244|PubMed:19369195}; propagated from UniProtKB/Swiss-Prot (Q99570.3)" Site 865 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine. {ECO:0000244|PubMed:19369195}; propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 985..1269 /region_name="WD40" /note="WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from...; cl02567" /db_xref="CDD:295369" Region 991..1358 /region_name="WD40" /note="WD40 repeat [General function prediction only]; COG2319" /db_xref="CDD:225201" Region 991..1030 /region_name="WD 1" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Site order(992,1010,1014,1020..1021,1039..1040,1059,1063, 1069..1070,1090..1091,1104,1113,1118,1124..1125,1137,1158, 1162,1168..1169,1182..1183,1201,1206,1213..1214, 1226..1227,1258,1262,1268..1269) /site_type="other" /note="structural tetrad" /db_xref="CDD:238121" Region 997..1040 /region_name="WD40 repeat" /note="WD40 repeat [structural motif]" /db_xref="CDD:293791" Region 1040..1079 /region_name="WD 2" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 1045..1091 /region_name="WD40 repeat" /note="WD40 repeat [structural motif]" /db_xref="CDD:293791" Region 1093..1134 /region_name="WD 3" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 1099..1136 /region_name="WD40 repeat" /note="WD40 repeat [structural motif]" /db_xref="CDD:293791" Region 1139..1178 /region_name="WD 4" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 1144..1179 /region_name="WD40 repeat" /note="WD40 repeat [structural motif]" /db_xref="CDD:293791" Region 1182..1223 /region_name="WD 5" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 1187..1236 /region_name="WD40 repeat" /note="WD40 repeat [structural motif]" /db_xref="CDD:293791" Region 1237..1278 /region_name="WD 6" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 1242..1268 /region_name="WD40 repeat" /note="WD40 repeat [structural motif]" /db_xref="CDD:293791" Site 1316 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphothreonine. {ECO:0000244|PubMed:18691976}; propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region 1327..1358 /region_name="WD 7" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99570.3)" Region <1327 ..1358="" 1..1358="" 1021="" 1081="" 1141="" 1201="" 121="" 1261="" 1321="" 1332..1357="" 181="" 1="" 241="" 301="" 361="" 39="" 421="" 481="" 541="" 555-560="" 601="" 61="" 661="" 721="" 781="" 841="" 901="" 961="" a="" adfnyffdts="" akqmmenaew="" and="" apflchpnlw="" aqddativrl="" aqnclpfqka="" arakhreglv="" associated="" ayaenialla="" aypersyvva="" cds="" chronic="" cmcqlgllqk="" coded_by="NM_014602.2:582..4658" complex="" core="" cqgshylaia="" csndgtvkiw="" db_xref="MIM:602610" ddkrarkhvk="" dgivkvwk="" dgnpilltag="" dlpekaegep="" dqkedgcvvd="" eeeedkllal="" ehksavnrir="" eleymrdpst="" erilvirkdl="" erklvllsvl="" eskppppgwr="" etalrflelv="" expression="" eyilpgiahl="" faketflsad="" gene="PIK3R4" gene_synonym="p150; VPS15" genes="" gniihnlcgh="" gpsddtprrg="" gstsspsvsy="" hdssqkgvid="" hffpeqvlnk="" high="" hpngnydtel="" hpsrarirrl="" href="https://www.ncbi.nlm.nih.gov/pubmed/25840748" iaavqgnnev="" ieasklpksp="" iedhsirelv="" ifsagcviae="" iggrvktltf="" ihqcwlcigt="" iitdvatfqt="" in="" ipvlsstilp="" irygavgfit="" is="" itrlcvffgr="" its-andersen="" ivgvaayvgw="" ivkqtlmeng="" journal="" kanivdqshl="" kdfmmksnka="" kenglvilvs="" kepvsrsifd="" kihplqsril="" kptylpednp="" kreqcnaeri="" krngslpdcp="" ksgvrhgdik="" ktelqqliqq="" laalgitgrq="" leelkirlns="" leuk.="" leukemia="" lfrhlhmrqk="" lfrqyvrdnl="" lftegvplfd="" lilhlaprls="" lkkllsqgmt="" lsqllayrng="" ltkvlalvke="" lvgwdlrsss="" lymphocytic="" mb.="" mgnqlagiap="" mhhfnsgaqs="" mitflndknd="" moller="" mpqalpkgsd="" nawtlkhdlk="" nniekrwiaf="" note="WD40 repeat [structural motif]" nsqkmegktt="" of="" origin="" peslpvghhd="" phvyefasdi="" pi3k="" pkgllvahlh="" plselqpsph="" plstssqvpe="" plvdlnsnqr="" pnneeidevt="" poor="" ppedpaiaql="" prognosis="" prvraealrt="" pubmed="" pyitqpiiqi="" qalhemvqqk="" qdsnvneewk="" qeviqtgkpp="" qiltavdqah="" qkandvllsh="" qlknlnmend="" qqglsdaeef="" qrgnafpeif="" qsssilkpll="" region="" region_name="WD40 repeat" res.="" rfqlpisshc="" rftlwassap="" rfvdggmfat="" rleaedylkq="" rrrtcyiape="" rsessagicv="" sdihdfeydk="" sdmkirfwdl="" sdngavqllg="" sekasekaam="" sglitsfavd="" slgstrffkv="" smfgsldppn="" smhplyqswv="" smwdmetgdr="" sqilsvesyf="" ssgtmacwdm="" styqirittc="" svhgiycspa="" t="" tenvmvtswn="" title="" tlpltsykqe="" tqgfivtasr="" tqmihrepdk="" trgelkramd="" ttrsiltysr="" vdlvktkqep="" veilldritp="" vitsclqtlk="" vivkalyalt="" vlayatvngs="" vprndiniyp="" vqeiqnkqkv="" vsdehslfat="" vttvqnkkpv="" vvarqistad="" vvkvfaiqdp="" vvtllsdpen="" vycklmpyld="" whlrgaffds="" with="" wvlltdfasf="" yalrskdits="" ycdsklaale="" ydristrpfl="" yllhfsndsv="" yrkiiegtev="" ytflqpymaq="">25840748
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