NUDT21 (16q13), CFIM, CPSFS , cleavage and polyadenylation specifity factor subunit 5

https://www.ncbi.nlm.nih.gov/gene/11051
 The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008]

Preferred Names

cleavage and polyadenylation specificity factor subunit 5

Names

CPSF 25 kDa subunit

cleavage and polyadenylation specific factor 5, 25 kD subunit

cleavage and polyadenylation specific factor 5, 25 kDa

cleavage and polyadenylation specificity factor 25 kDa subunit

cleavage factor Im complex 25 kDa subunit

nucleoside diphosphate-linked moiety X motif 21

nudix (nucleoside diphosphate linked moiety X)-type motif 21

nudix motif 21

pre-mRNA cleavage factor Im (25kD)

pre-mRNA cleavage factor Im 25 kDa subunit

pre-mRNA cleavage factor Im 68 kDa subunit

pre-mRNA cleavage factor Im, 25kD subunit

Related articles in PubMed

1. NUDT21 regulates 3'-UTR length and microRNA-mediated gene silencing in hepatocellular carcinoma. Sun M, et al. Cancer Lett, 2017 Dec 1. PMID 28964783
2. The crystal structure of human cleavage and polyadenylation specific factor-5 reveals a dimeric Nudix protein with a conserved catalytic site. Trésaugues L, et al. Proteins, 2008 Dec. PMID 18767156
3. Crystal structure of the 25 kDa subunit of human cleavage factor Im. Coseno M, et al. Nucleic Acids Res, 2008 Jun. PMID 18445629, Free PMC Article
4. RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins. Ray D, et al. Methods, 2017 Apr 15. PMID 27956239, Free PMC Article
5. MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma. Zhu ZJ, et al. Mol Med Rep, 2016 Nov. PMID 27633853

See all (81) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

1. RNA sequence preferences of unconventional RNA-binding proteins, Nudt21 and CNBP, has been described.
2. Loss of NUDT21 shortened the 3'-UTR of various oncogenes in hepatocellular carcinoma cells. The shorter 3'-UTR contained less miRNA binding sites, which enabled the oncogenes to evade miRNA regulation and become overexpressed in HCC, leading to unregulated cancer cell proliferation.
3. it was demonstrated that the CFIm25 protein was also downregulated in osteosarcoma tissues, and inhibited the proliferation and promoted the apoptosis of the cells. Elucidating the roles of miR181a and CFIm25 in osteosarcoma not only assists in further understanding the pathogenesis and progression of this disease, but also offers novel targets for effective therapies.
4. The authors identify NUDT21 as a novel candidate for intellectual disability and neuropsychiatric disease, and elucidate a mechanism of pathogenesis by MeCP2 dysregulation via altered alternative polyadenylation.
5. Structure of a CFI(m)25/CFI(m)68 RRM heterotetramer and biochemical data indicated that CFIm25 specifically recognized two UGUA elements, CFIm68 facilitates looping of the intervening RNA. CFIm-mediated RNA looping may regulate alternative polyadnylation.
6. Crystal structure of CFIm25-CFIm68 RRM heterotetramer illustrated that CFIm25 homodimer is clamped by two CFIm68 monomers on each side of the dimer interface.
7. Data provide evidence that CFIm exists as a heterotetramer of 25-kD, 59-kD and 68-kD subunits of CFIm: CFIm25, CFIm59 and CFIm68.
8. crystal structures of the CFI(m)25 homodimer in complex with UGUAAA and UUGUAU RNA sequences
9. Crystallographic and biochemical experiments suggest that CF I(m)25 specifically recognizes UGUA RNA sequence and binds to two UGUA simultaneously
10. The crystal structure of human CPSF5 was solved at 1.9 A resolution.

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